Advances in Applied Biotechnology: Proceedings of the 2nd by Tong-Cun Zhang, Motowo Nakajima

By Tong-Cun Zhang, Motowo Nakajima

At the ICAB 2014, researchers from world wide will assemble to debate the newest medical learn, findings and applied sciences touching on Microbial Genetics and Breeding, Optimization and keep watch over of organic tactics, organic Separation and organic Purification, and Advances in Biotechnology.
This convention will offer a platform for tutorial alternate at the software of biotechnology among family and foreign universities, learn institutes, company specialists and students. The members will concentrate on the foreign improvement and destiny tendencies. the development will lay an exceptional origin for addressing key technical demanding situations in quite a few components of utilized biotechnology, delivering possibilities to advertise the advance and enlargement of the biotechnology industry.

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DNA barcode technology was a powerful tool for Microcystis identification. Acknowledgments This work was supported by The International Science & Technology Cooperation Program of China (Grant No. 2013DFA71340) and Major National Science & Technology Project of Water Pollution Control and Management of China (Grant No. 012ZX07203-002). References 1. An J, Carmichael WW (1994) Use of a colorimetric protein phosphatase inhibition assay and enzyme linked immunosorbent assay for the study of microcystins and nodularins.

Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor 11. Kuroda S, Tanizawa K, Sakamoto Y, Tanaka H, Soda K (1990) Alanine dehydrogenase from two Bacillus species with distinct thermo stabilities: molecular cloning, DNA and protein sequence determination, and structural comparison with other NAD (P)-dependent dehydrogenases. Biochemistry 29:1009–1015 12. Ohshima T, Nishida N, Bakthavatsalam S, Kataoka K, Takada H, Yoshimura T, Esaki N, Soda K (1994) The purification, characterization, cloning and sequencing of the gene for a halo stable and thermo stable leucine dehydrogenase from Thermo actinomyces intermedius.

5 HPLC result of the standard L-tert-Leucine (a) and product of the reaction system (b) 28 J. Bai et al. Fig. 3 Identification of L-tert-Leucine The reaction liquid was detected by HPLC (Fig. 5) and LC-MASS (Fig. 6). The results show that the appearance time of product was the same as the standard substance at 21 min (Fig. 5). 1 (−) and was identical to the standard substance. 4 Discussion In this study, we successfully constructed L-tert-Leucine producing strains by introducing leudh from B. cereus and fdh from C.

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