By Elke Hahn?Deinstrop(auth.)
Thin-layer chromatography (TLC) is a strong, quick and cheap analytical technique. It has confirmed its usefulness in pharmaceutical, meals and environmental research. This re-creation of the sensible TLC consultant incorporates a thoroughly revised bankruptcy on documentation, now together with using electronic cameras. chosen new sorbents and tools also are introduced.
Why has the earlier variation been winning? All steps of the analytical technique are truly defined, beginning with the alternative of an acceptable TLC process and finishing with information overview and documentation. unique emphasis is wear the right kind collection of fabrics for TLC. homes and services of varied fabrics and the TLC gear are defined, overlaying e. g. precoated layers, solvents and constructing chambers, together with info on providers. Many functional tricks for difficulty taking pictures are given. All this is often illustrated with a variety of colored figures.
the way to use TLC in compliance with GLP/GMP laws is defined intimately, together with the mandatory documentation. consequently the reader can with ease collect his personal regular working procedures.Content:
Chapter 1 creation (pages 1–13):
Chapter 2 Precoated Layers (pages 15–33):
Chapter three sooner than the TLC improvement approach (pages 35–68):
Chapter four Solvent structures, constructing Chambers and improvement (pages 69–112):
Chapter five review with out Derivatization (pages 113–123):
Chapter 6 Derivatization (pages 125–151):
Chapter 7 review After Derivatization (pages 153–180):
Chapter eight Documentation (pages 181–201):
Chapter nine GMP/GLP?Conforming Operations in TLC (pages 203–239):
Chapter 10 results of rigidity (pages 241–245):
Chapter eleven precise tools in TLC (pages 247–252):
Chapter 12 Appendix (pages 253–270):
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Extra resources for Applied Thin-Layer Chromatography: Best Practice and Avoidance of Mistakes, Second Edition
G sample substance 296 Photograph Section Figure 71. 2 “Two-dimensional Thin-Layer Chromatography” V 1 = Comparison 1 (birch leaves preparation, not hydrolyzed) V 2 = Comparison 2 (aglyca, applied after hydrolysis of part of the plate) Figure 72. 1) Photograph Section J—1 J ' 1 Figure 73. Evaluation of chromatograms of two active ingredients with different absorption properties in short-wave UV light (a) Shot of the TLC plate in 254-nm UV light before the derivatization (b) Shots in white light after treatment in the iodine vapor chamber for 16 h Left-hand part of the plate: Pointwise applied sample solutions, 10 ?
025 ? g ref. , lanes 5 + 6 each 25 ? g sample substance 296 Photograph Section Figure 71. 2 “Two-dimensional Thin-Layer Chromatography” V 1 = Comparison 1 (birch leaves preparation, not hydrolyzed) V 2 = Comparison 2 (aglyca, applied after hydrolysis of part of the plate) Figure 72. 1) Photograph Section J—1 J ' 1 Figure 73. Evaluation of chromatograms of two active ingredients with different absorption properties in short-wave UV light (a) Shot of the TLC plate in 254-nm UV light before the derivatization (b) Shots in white light after treatment in the iodine vapor chamber for 16 h Left-hand part of the plate: Pointwise applied sample solutions, 10 ?
G. 3 Where Is TLC Used? 3 Where Is TLC Used? Pharmaceuticals and Drugs Identification, purity testing and determination of the concentration of active ingredients, auxiliary substances and preservatives in drugs and drug preparations, process control in synthetic manufacturing processes. Clinical Chemistry, Forensic Chemistry and Biochemistry Determination of active substances and their metabolites in biological matrices, diagnosis of metabolic disorders such as PKU (phenylketonuria), cystinuria and maple syrup disease in babies.