By L. G. Koss (auth.), Prof. Dr. med., Dr. rer. nat. Peter Pfitzer, Prof. Dr. med. E. Grundmann (eds.)
Progress in technological know-how is frequently promoted by way of a brand new process. Diagnostic cytology, despite the fact that, constructed slowly over a complete century, commonly in differentiating malignant from benign cells from numerous tissues. The decisive step during this improvement was once the in depth research of 1 neighborhood ization via Papanicolaou: the applying of cytology for screening within the box of gynecologic oncology made it an stated process. accordingly, fabrics inves tigated ahead of have been verified back on a bigger scale and new ones have been incorporated into this system. the potential of a wide-range software of this diag nostic procedure, which consists of a low chance for the sufferer and is within your means, attracted the experts of many fields. one of many difficulties which has resulted is the coordination of teaching and caliber insurance for a wide crew of individuals from diversified fields and with varied pursuits and whose adventure in morphology varies. during this quantity normal difficulties of cytology are dis stubborn, as is the query "who is a clinical cytologist?" schooling and coaching are the subjects of the contributions through Coleman, Holzner, Jenny, Koss and Muller, disguise ing the placement within the eu neighborhood, Germany, Austria, Switzerland, and the united states. a different contribu tion by means of Lange matters the location of cytotechnologists, paramedicals very important for cytologic screening programs.
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Extra resources for Current Status of Diagnostic Cytology
When using hybridization techniques for clinical purposes, one should demand high specificity and reasonable sensitivity of the method chosen. The application of HPV hybridization techniques should be restricted to highly experienced laboratories which have different methods available that are suited to the special problem of clinical concern. Recent Results in Cancer Research, Vol. 133 © Springer-Verlag Berlin' Heidelberg 1993 Immunocytochemistry in Diagnostic Cytology P. Dalquen, G. Sauter, R.
9%) for tumor typing; in 65 cases to differentiate mesothelioma from adenocarcinoma; and in 147 cases, mainly effusions, for the differentiation between benign (reactive) and malignant lesions (Table 2). Methods Fixation Smears of fine-needle aspiration and brush biopsies were usually fixed with commercially available spray containing polyethyleneglycol in ethanol. 5 mIl 1000 1 M trichloric acetic acid). Cytologic Staining Method The smears were usually stained with Papanicolaou's stain. The results of immunocytochemistry do not depend on the staining method (Li et al.
However, the incubation time can be reduced to a few minutes so that no background staining occurs. Immune peroxydase (peroxidase-anti-peroxidase - PAP, ABC method) and immune alkaline phosphatase techniques (immune alkaline phosphatase - lAP; alkaline phosphatase-anti-alkaline phosphatase - APAAP) are especially suitable to cytology as cytologic details of normal versus malignant cells remain clearly recognizable and the staining product is stable. To circumvent the problem of endogenous peroxidase, some authors prefer alkaline phosphatase techniques (To et al.