Elastic Filaments of the Cell by Károly Trombitás (auth.), Henk L. Granzier, Gerald H.

By Károly Trombitás (auth.), Henk L. Granzier, Gerald H. Pollack (eds.)

Elastic filaments refer quite often to titin, the most important of all identified proteins. Titin was once stumbled on firstly in muscle cells, the place it interconnects the thick filament with the Z-line. Titin varieties a molecular spring that's liable for protecting the structural integrity of contracting muscle, making sure effective muscle contraction. extra lately, it has develop into transparent that titin isn't constrained to muscle cells on my own. for instance, titin is located in chromosomes of neurons and likewise in blood platelets. This subject is speedy turning into a focus for examine in figuring out viscoelastic homes on the molecular, mobile, and tissue degrees. In titin could lie a known foundation for organic viscoelasticity. It has develop into transparent that titin may well carry the major to definite medical anomalies. for instance, it's transparent that titin-based ventricular stiffness is modulated via calcium and that titin is answerable for the altered stiffness in cardiomyopathies. it's also transparent from proof from a bunch of Finnish households that titin mutations could underlie a few muscular dystrophies and that with different mutations chromatids fail to split in the course of mitosis. therefore, it's transparent that this protein may have vital medical implications stemming from its biomechanical function. One element of this box is the bringing jointly of bioengineers with medical researchers and biologists. Genetic and biochemical elements of titin-related proteins are being studied including front-line engineering techniques designed to degree the mechanics of titin both in small aggregates or in unmarried molecules.

Show description

Read Online or Download Elastic Filaments of the Cell PDF

Similar nonfiction_9 books

Genetic Engineering: Principles and Methods

Genetic Engineering: ideas & MethodsJane ok. Setlow, PhDBrookhaven nationwide Laboratory, Upton, big apple, USAAbout the Series:Genetic Engineering: ideas and strategies provides state of the art discussions in sleek genetics and genetic engineering. fresh volumes have lined gene treatment study, genetic mapping, plant technological know-how and know-how, shipping protein biochemistry, and viral vectors in gene remedy, between many different themes.

The Welfare of Animals: The Silent Majority

The standard of lifestyles that we offer for animals for nutrition, companionship, game and garments will be certain their welfare, or even the welfare of untamed animals is suffering from human actions. This booklet demanding situations us to mirror on that silent majority of animals without voice. we're more and more wondering no matter if our use of animals is important, fascinating and humane, The booklet offers a framework to make these tough judgements.

Safety, Reliability and Risk Analysis: Theory, Methods and Applications

Protection, Reliability and chance research. concept, equipment and functions comprises the papers provided on the joint ESREL (European safeguard and Reliability) and SRA-Europe (Society for hazard research Europe) convention (Valencia, Spain, 22-25 September 2008). The e-book covers a variety of themes, together with: twist of fate and Incident research; challenge and Emergency administration; selection aid structures and software program instruments for protection and Reliability; Dynamic Reliability; Fault identity and Diagnostics; Human elements; built-in possibility administration and Risk-Informed Decision-making; Legislative dimensions of hazard administration; upkeep Modelling and Optimisation; Monte Carlo tools in process safeguard and Reliability; Occupational defense; Organizational studying; Reliability and safeguard facts; assortment and research; threat and proof dependent coverage Making; possibility and danger research; chance keep an eye on in advanced Environments; possibility belief and verbal exchange; protection tradition; defense administration platforms; software program Reliability; Stakeholder and public involvement in chance governance; Structural Reliability and layout Codes; approach Reliability research; Uncertainty and Sensitivity research.

Extra info for Elastic Filaments of the Cell

Sample text

Elastic properties of titin filaments demonstrated using a freeze-fracture technique. Cell Motil CytoskelI993;24:274-283 Trombitas K, Jin Jp, Granzier H. The mechanically active domain of titin in cardiac muscle. eirc Res 1995;77:856-861. Trombitas K, Greaser M, Labeit S, Jin JP, Kellermayer M, Helmes M, Granzier H. Titin extensibility in situ: entropic elasticity of permanently folded and permanently unfolded molecular segments. J Cell BiolI998a;140:853-859. Trombitas K, Greaser M, French G, Granzier H.

Fragments were extracted from an agarose matrix by using the Jetsorb kit (Genomed, Bad Oyenhausen, Germany). 8 x lOx Y-Tango buffer (MBI Fermentas, Vilnius, Lithunia). After incubation for 2 hours at 37°C, restriction enzymes were inactivated by a phenol extraction, excess phenol was removed by an isopropanol precipitation, followed by a 70% ethanol wash. Obtained fragments were then ligated into a pET9D vector (Studier and Moffat, 1986) which was modified to express their insert sequences as N-terminal His6tags fusions.

The following libraries have been amplified and aliquots are being used for titin subclone construction: human cardiac cDNA library in larnbda-ZapII vector backbone (Stratagene #936208), human skeletal cDNA library in gtlO Centner, Fougerousse, Freiburg, Witt, Beckmann, Trombittis, Gregorio, and Labeit 39 (Clontech, Cat. # HL1024) and human skeletal muscle cDNA library in pGADlO (Clontech, Cat. # HL4010AB). This latter library (with cDNA inserts in the plasmid vector) has become our "default template" because the amplification efficiency of the PCR reaction is higher in comparison with cDNA pools inserted into the 50 kb GC-rich lambda phage vector backbone.

Download PDF sample

Rated 4.58 of 5 – based on 17 votes