By Harry W. M. Steinbusch, S. Peter Van Vliet, John G. J. M. Bol, Jan De Vente (auth.), André Calas, Daniel Eugène (eds.)
The nice strides made within the box of morphological equipment in past times many years have possibly discovered their so much incredible expression within the practical exploration of the anxious process. compared to different tissues, nerve tissue monitors 3 varieties of specificity : structural, as a result of designated association of the neuronal networks ; chemical as proven, for instance, via the informative molecules exchanged among the nerve cells, and naturally sensible, because of the actual metabolic and electrophysiological features of the neurons. even if for a very long time the structural houses of the fearful method have been normally thought of to represent the one box to which morphological strategies can be utilized, we're to-day justified in believing that they could additionally discover the nerve tissue via its particular chemical and sensible facets, due to the improvement of immunocytochemistry and in situ hybridization, to the elaboration of the deoxyglucose strategy, to using voltage or ion delicate dyes, and to the development made within the software of in vivo ideas like puppy. those tools have developed so quickly, the technical and basic difficulties they increase are so a number of and stimulating, and the significance of the complementary facts they supply is so visible, that we idea it was once a great time to prepare a brand new assembly among wonderful experts within the neurocytochemical field.
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Extra info for Neurocytochemical Methods
1986). The problem of matching antigen preparations and reaction products in tissue may account for the observation that monoclonal antibodies recognizing the antigen preparation in the selection procedure may not yield immunohistochemical staining patterns. , 1989). Thus, it seems that our antigen preparations contain antigenic determinants that are similar enough to those present at the sites relevant for the localization of immunoreactivity for a certain amino acid in tissue. The antigen preparations, the immunization schedules, the hybridoma production and the selection procedure involving an ELISA system have been described in detail by Matute and Streit (1986) and by Liu and collaborators (1989).
1989c). In the two models systems, the nitrocellulose strip (Larsson 1982) and the gelatin model (Schipper and Tilders 1983) we demonstrated the specificity of our antibodies towards other formaldehyde-fixed nucleotides and nucleosides (De Vente et al. 1987,1989c) and we also showed the Steiner antibodies were much less efficient in binding to formaldehyde-fixed cGMP (De Vente et al. 1989c). Glutaraldehyde is a preferred fixative for the demonstration of monoamines and amino acids and gives a good tissue fixation (Geffard et al.
Neuropil labeling in surrounding of labeled neurons. Bars: 5mm in A, 1 mm in B, 200 JJm in C and 0, 30 Jjm in E. , 1987; reproduced with permission) Figure 2A-E (next page): Immunohistochemical labeling patterns in semithin sections from perfusionfixed brain tissue. A: Staining pattern in part of rat cerebral cortex obtained with monoclonal 'anti-GABA' antibody. Labeled terminal-like elements surrounding perikaryon of pyramidal neuron in layer 5 (arrowheads). Immunoreactive cell bodies of interneurons (arrows).