Biotechnology of Aroma Compounds (Advances in Biochemical by R.G. Berger, Jan A.M.de Bont, P.S.J. Cheetham, R. Croteau,

By R.G. Berger, Jan A.M.de Bont, P.S.J. Cheetham, R. Croteau, I.L. Gatfield, J.-B. Gros, C. Larroche, D.J. Leak, D. McCaskill, P. Schreier, A.H. Scragg, G.K. Skouroumounis, M.J. van der Werf, P. Winterhalter

Discussing the biotechnology of aroma compounds, this paintings comprises assurance of: enzymes and flavour biotechnology; glycoconjugated aroma compounds - occurence, function and biotechnological transformation; and customers for the bioengineering of isoprenoid biosynthesis.

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Biotechnology of Aroma Compounds (Advances in Biochemical Engineering Biotechnology)

Discussing the biotechnology of aroma compounds, this paintings contains insurance of: enzymes and flavour biotechnology; glycoconjugated aroma compounds - occurence, function and biotechnological transformation; and clients for the bioengineering of isoprenoid biosynthesis.

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Haffner and Tressl (1996) have investigated by the biosynthesis of (R)-7-decalactone by Sporobolomyces odorus involving a strictly enantioselective (R)-12-hydroxylation of oleic acid, followed by 13-oxidation and then lactonisation. Also, Gatfield (1995) has mentioned that in the conversion of the 2-methyl butanol present in fusil oil by Acetobacter aceti, into the corresponding 2-methylbutannoic acid, very little racemisation takes place, which is surprising as it is well known that this bioconversion is a two-step process which proceeds via the intermediate aldehyde, which is well known to undergo facile tautomerisation.

The application of biocatalyst technology to terpenes has been slow, perhaps because of their poor water solubility and biocidal properties, but also because of the easy availability of many terpenes by simple extraction from plant sources. Nevertheless some good examples exist. These include a high yielding biotransformation of limonene into a-terpineol by selective hydration [38]. , by means of cysteine addition across the double bond of the pulegone followed by the use of specially selected [3-1yase to cleave the S-C bond to produce the sulphur-containing terpene [17].

G. g. from Brevibacterium ammoniagenes Table 13. Bioengineering aspects of bioflavour production 7-Decalactone Benzaldehyde precursors Vanillin Soy sauce Milk souring Hexanal Ethyl isovalerate Monosodium glutamate The precursor is an oil and so water-miscibility issues had to be solved. Toxic HCN is produced when using cyanogenic nitrile glycoside. A complex pathway is involved. When eugenol is used as the precursor then precursor toxicity to the cells has to be dealt with. Several strains of microorganism have been used, separately immobilised and operated in series.

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